ABSTRACT
Objective To investigate the interference of blood urine on red cell detection of subsequent adjacent specimen on Sysmex UF-1000i flow cytometer urine sediment analyzer and to propose some countermeasures.Methods Totally 60 copies of fresh urine specimens having the results in normal range were chosen,and then each copy was divided into three pieces.All the pieces of specimens were put on the tube rack between the low-,medium-and high-concentration blood urine,and the urine was in front of the specimens.UF-1000i automatic urinary sediment analyzer was used for detection under auto sampling mode,and paired t-test was carried out for the results before and after interferences by concentrations of blood urine.The carryover was measured for the specimens and blood urine,and the specimens underwent microscopy.Results The RBC results had no significant difference before and after the interference by low-concentration blood urine (P＞0.05),while had statistical differences before and after the interferences by medium-and high-concentration blood urine (P＜0.01).There was also obvious difference between the RBC results interfered by medium-concentration blood urine and by highconcentration one.There were no significant differences between the results of WBC,epithelial cell and cast before and after the interferences (P ＞0.05).The urinary sediment microscopy results were within the normal range,and the highconcentration blood urine had the carryover gain the maximum value.Conclusion UF-1000i automatic urinary sediment analyzer has the RBC result higher than the actual one after the interference,and the interference deteriorates with the increased concentration of blood urine.The blood urine specimen has to be tested separately,and deep cleaning has to be performed for the analyzer.The test by the analyzer had better be combined with microscopy to avoid carryover.
ABSTRACT
Objective To investigate the interference of blood urine on red cell detection of subsequent adjacent specimen on Sysmex UF-1000i flow cytometer urine sediment analyzer and to propose some countermeasures.Methods Totally 60 copies of fresh urine specimens having the results in normal range were chosen,and then each copy was divided into three pieces.All the pieces of specimens were put on the tube rack between the low-,medium-and high-concentration blood urine,and the urine was in front of the specimens.UF-1000i automatic urinary sediment analyzer was used for detection under auto sampling mode,and paired t-test was carried out for the results before and after interferences by concentrations of blood urine.The carryover was measured for the specimens and blood urine,and the specimens underwent microscopy.Results The RBC results had no significant difference before and after the interference by low-concentration blood urine (P＞0.05),while had statistical differences before and after the interferences by medium-and high-concentration blood urine (P＜0.01).There was also obvious difference between the RBC results interfered by medium-concentration blood urine and by highconcentration one.There were no significant differences between the results of WBC,epithelial cell and cast before and after the interferences (P ＞0.05).The urinary sediment microscopy results were within the normal range,and the highconcentration blood urine had the carryover gain the maximum value.Conclusion UF-1000i automatic urinary sediment analyzer has the RBC result higher than the actual one after the interference,and the interference deteriorates with the increased concentration of blood urine.The blood urine specimen has to be tested separately,and deep cleaning has to be performed for the analyzer.The test by the analyzer had better be combined with microscopy to avoid carryover.
ABSTRACT
Objective To evaluate the prevalence of depression,anxiety and suicide behavior in patients suffering from tuberculosis in Hangzhou and to explore their relationship with medication adherence. Methods Demographic characteristics,self-rating anxiety scale (SAS),the center for epidemiological studies -depression (CESD),social support rating scale (SSRS),suicide behavior information and the morisky medication adherence scale (MMAS)were investigated in 973 tuberculosis patients who were selected by systematic random sampling.Results The means of SAS and CESD were 39.71 ±8.30 and 14.16 ±10.77 respectively,which were both higher than the norms(P<0.01).Totally 102 (10.48%)patients had anxiety and 333 (34.22%)were depressed.Out of 973 patients,60 (6.17%)reported suicide ideation after tuberculosis diagnosis.The prevalence of non -adherence was 20.55%,which was defined with MMAS score above one and more.The non -adherence group had higher anxiety,depression and suicide ideation prevalence than the adherence group (15.50%vs.9.18%,46.50%vs.30.66%,11.00%vs.4.92%respectively,P<0.01).The mean score of SSRS,subjective support,objective support and utilization of support in the non-adherence group were 30.71 ±5.15,4.61 ±2.07,19.74 ±4.55 and 6.34 ±1.93 respectively,which were 34.06 ±7.39,6.62 ± 2.27,20.67 ±5.27 and 6.77 ±2.23 in the adherence group respectively.SSRS and its three dimension scores were significantly lower in the non-adherence group than that in the adherence group (P<0.01).Conclusion These findings show a quite serious situation of psychological problems of tuberculosis patients in Hangzhou and suggest psychological intervention should be included in adherence intervention.